ABSTRACT
Objective::To investigate the chemical constituents from Paeonia veitchii. Method::P. veitchii samples (30 kg) were extracted with 95% ethanol for four times and then filtrated, and the combined filtrates were concentrated under vacuum to get the extracts. After suspension with water, exaction was conducted with petroleum ether, dichloromethane, ethyl acetate, n-butanol and water successively to obtain five corresponding fractions. The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS column chromatography and prep-HPLC, and the structures of these compounds were determined by such spectrum technologies as infrared spectroscopy (IR), mass spectroscopy (MS), and nuclear magnetic resonance (NMR). Result::Sixteen compounds were isolated and their structures were identified as follows (1S, 5R, 6R)-1, 8-dihydroxypin-2-en-4-one (1), (2-hydroxyl)-phenyl-methyl-β-D-xylopyranoside (2), flufuran (3), 6′-O-vanillylpaeoniflorin (4), methyl 2, 5-dihydroxycinnamate (5), (1S, 2S, 5R, 6R)-1, 8-dihydroxypin-4-one (6), palbinone (7), 4-O-methylpaeoniflorin (8), 4-O-ethylpaeoniflorin (9), benzoyloxypaeoniflorin (10), benzoic acid (11), gallic acid (12), methyl gallate (13), ethyl gallate (14), β-sitosterol (15), and 1, 2, 3, 4, 6-penta-O-galloyl-β-D-glucopyranose (16). Conclusion::Compounds 1, 2 were new natural compounds; compound 3 was isolated from genus Paeonia for the first time, and compounds 4-7 were isolated from this plant for the first time.
ABSTRACT
Objective: To study the chemical constituents from the root bark of Litsea glutinosa. Methods: Silica gel and Sephedex LH-20 column chromatographies as well as semi-preparative HPLC were applied to isolate and purify the compounds. Their structures were elucidated based on the spectrum analysis. Results: Fifteen compounds were obtained and identi-fied as schizandriside (1), lyonside (2), coclaurine (3), alangisesquin A (4), alangisesquin B (5), dihydrobuddlenol (6), ssioriside (7), dendranthemoside B (8), epi-anhydrocinnzeylanol (9), isolariciresinol-5’-methoxy-iso-larixa-9’-O-β-D-xylopyranoside (10), benzyl alcohol-β-D- glucopyranoside (11), phenylethyl-β-D-glucopyranoside (12), n-butyl-β-D-fructoside (13), N-cis-feruloyl tyramine (14), and N-trans-sphingoyl tyramine (15). Conclusion: Compounds 1-13 are isolated from this plant for the first time.
ABSTRACT
Objective To study the chemical constituents from the rhizomes of Cimicifuga dahurica. Methods The chemical constituents from the rhizomes of C. dahurica in ethyl acetate extraction phase and H2O fraction were isolated and purified by chromatographic methods, such as silica gel, opening ODS column, Sephadex LH-20, ODS and semi-preparative HPLC. Which stucture were identified by NMR and physicochemical analysis. Results Fifteen compounds were isolated and identified as 20(R),23 (R),24(R),25(S),26(S)-16β:23; 23:26; 24:25-triepoxy-12β-acetoxy-3β,26-dihydroxy-9,19-cyclolanost-7-ene-3-O-β- D-xylopyranoside (1), cimiaceroside B (2), 23-O-acetylshengmanol-3-O-β-D-xylopyranoside (3), 7,8-didehydro-24-O-acetylhydroshengmanol-3-O-β-D- xylopyranoside (4), 24-epi-cimigenol-3-O-β-D-xylopyranoside (5), 24-O-acetyldahurinol-3-O-β-D-xylopyranoside (6), 24-epi-24-O- acetylhydroshengmanol-3-O-β-D-xylopyranoside (7), 12β-O-acetylcimigenol-3-O-β-D-xylopyranoside (8), 23-O-acetyl-7,8- didehydroshengmanol-3-O-β-D-xylopyranoside (9), 7,8-didehydro-25-dehydrocimigenol-3-O-β-D-xylopyranoside (10), 25-O- ethylcimigenol-3-O-β-D-xylopyranoside (11), 3,4-dihydroxybenzoic acid (12), 24-O-acetylisodahurinol-3-O-α-L-arabinopyranoside (13), 2,3-dihydroxy-2-[(4-hydroxyphenyl) methyl]-1-ethyl ester (14), and 2,3-dihydroxy-2-[(4-hudroxyphenyl) methyl]-4-ethyl ester (15). Conclusion Compounds 6, 11-12, and 14-15 are isolated from Cimicifuga genus for the first time. Compounds 1, 4-5, 8-10, and 13 are isolated from C. dahurica for the first time.
ABSTRACT
Objective: To study the chemical constituents of n-butanol fraction from the stems of Schisandra chinensis. Methods: The constituents were separated and purified by chromatographic technology, which structures were elucidated by physicochemical constants and spectral data analyses. Results: Fourteen chemical constituents were isolated from the n-butanol extract in the stems of S. chinensis, and the structures were identified as quercertin (1), quercertin-3-O-β-D-glucopyranoside (2), quercetin-3-O-β-D-xylopyranoside (3), rutin (4), apigenin (5), apigenin-7-O-β-D-glucopyranoside (6), isorhamnetin-3-O-β-D-glucopyranoside (7), genistein-7-O-β-D-glucopyranoside (8), chlorogenic acid (9), ferulaic acid (10), caffeic acid (11), oleanolic acid (12), (+)-catechin-7-O-β-D-glucopyranoside (13), and daucosterol (14). Conclusion: The compounds 3, 7, 8, and 13 are obtained from this plant for the first time.
ABSTRACT
OBJECTIVE: To study the chemical constituents of Dendrobium officinale. METHODS: Various column chromatographic techniques were used to separate and purify the chemical constituents and their structures were elucidated by spectral analysis. RESULTS: Seven compounds were isolated and identified as apigenin-6, 8-di-C-β-S-O-glucopyranoside (I), apigenin-6-C-α-L-arabi-nopyranosyl- 8-C-β-D-xylopyranoside(II), apigenin-6-C-(2″-O-β-D- glucopyranosyl)-α-L-arabinopyranoside (III), apigenin-8-C-(2″-O-β-D- glucopyranosyl)-α-L-arabinopyranoside(IV), quercetin-3-O-β-D-rutinoside(V), D-sucrose(VI), and β-sitosterol-3-O-β-D-gluco-pyranoside (VII). CONCLUSION: The compounds I-V were isolated from Dendrobium officinale Kimura et Migo for the first time.
ABSTRACT
Objective: To study the chemical constituents in the barks of Pterocarya stenoptera. Methods: The chemical constituents in chloroform and ethyl acetate fractions of 90% ethanol extract from the barks of P. stenoptera were isolated and purified by column chromatography over silica gel, ODS, Sephadex LH-20 columns, preparative HPLC, and recrystallization. The structures of the isolated compounds were identified on the basis of physicochemical properties and spectral data analyses. Results: Fourteen compounds were obtained and identified as glochidone (1), lup-20(29)-en-1β, 3β-diol (2), ursolic acid (3), betulinic acid (4), 2α, 3β, 19α-trihydroxyurs-12-en-28-O-β-D-glucopyranoside (5), (4S)-4, 8-dihydroxy-1-tetralone (6), 5-hydroxy-4-methoxy-α-tetralone (7), (4S)-4-hydroxy-1-tetralone (8), myricitrin (9), 30-norlupan-28-oic acid (10), myrictin (11), quercetin-3-O-2″-galloyl-rhamnoside (12), oleanolic acid (13), and 3, 3'-di-O-methyl ellagic acid-4'-O-β-D-xylopyranoside (14). Conclusion: Compounds 1-14 are isolated from the plant for the first time, and compounds 5 and 7-14 are obtained from the plants in Pterocarya Kunth. for the first time.
ABSTRACT
Aim To study the chemical constituents of Rhododendron ovatum Planch. Methods The chemical constituents were isolated and purified by silica gel column chromatography and identified on the basis of their physiochemical and spectral data. Results Seven compounds were isolated and identified. Their structures were established as 3,5,7-trihydroxylchromone 3-O-β-D-xylopyranoside (I), taraxerol (II), β-sitosterol (III), betulinic acid (IV), quercetin (V), quercetin-3-O-α-L-rhamnopyranoside (VI), and D-glucose (VII). Conclusion Compound I is a new compound. Compounds II-VII were isolated from this plant for the first time.